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反向基因转染技术的最近研究进展
The Recent Development in Reverse Gene Transfection
随着基因治疗研究的深入,基因转染技术得到了长足的发展。在反向基因转染概念被提出来的十余年间,反向基因转染技术也引起了广泛关注。反向基因转染技术是相对于常规转染而言的,即首先通过基质锚定DNA(或RNA),然后将细胞接种于该基质表面,细胞贴附的同时摄取锚定的DNA(或RNA)而实现基因转染,因而也被称为表面介导基因转染、基质介导基因转染、固相基因转染。与传统转染方法相比,其具备以下优势:载体/DNA(或RNA)复合物更加稳定,转染试剂可以更有效地接触细胞而达到更高的转染效率,低的细胞毒性和血清环境中不影响转染效率等。本文主要综述了反向转染技术的最新研究进展及其主要应用领域。
With the in-depth study of gene therapy, the gene transfection techniques have achieved rapid development. Since it was proposed in 1997, reverse transfection concept has also aroused considerable attention. Reverse gene transfection is also known as surface-mediated transfection or matrix-mediated transfection, or solid-phase transfection. In a typical procedure of reverse transfection, DNA (or RNA) is firstly anchored to the substrate; cells are transplanted on the substrate surface in the next step; the adsorbed DNA (or RNA) is internalized into post-attached cells to realize the gene transfection. Compared with conventional gene transfection, the reverse method is imparted with several advantages including more stable complexation of vector/DNA (or RNA), more effective contact between the transfection reagent and cells, which is favourable for achieving higher transfection efficiency and lower cytotoxicity, and well-maintained transfection performance in the presence of serum. The recent research progress of reverse gene transfection and its applications are focused on in this review.
Contents
1 Introduction 2 Approaches to reverse gene transfection
2.1 Nucleic acid (and vector) directly anchored to the substrate surface
2.2 Electrostatic adsorption
2.3 Layer-by-layer
2.4 Hydrogen bond
2.5 Specific interaction
2.6 Covalent bond
3 Applications of reverse gene transfection
3.1 Tissue engineering
3.2 Gene screening
3.3 Primary cells
3.4 Non-adherent cells
4 Conclusion and perspective
reverse gene transfection
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