基于纳米材料的基因载体

古晓晓; 杜宝吉; 李云辉; 高莹*; 李丹; 汪尔康

doi:10.7536/PC150163

化学进展 ›› 2015, Vol. 27 ›› Issue (8) :1093-1101 DOI: 10.7536/PC150163

综述与评论

扫码查看或转发全文

基于纳米材料的基因载体

^1,2

作者信息 +

Vectors Based on Nanomaterials for Gene Delivery

Gu Xiaoxiao^1,2, Du Baoji², Li Yunhui¹, Gao Ying*¹, Li Dan*², Wang Erkang*²

Author information +

文章历史 +

摘要

基因疗法是治疗基因变异引起的先天性遗传疾病和后天获得性疾病以及癌症的新型有效方法。外源基因在细胞中安全、高效、稳定的表达是基因治疗成功的关键,这与基因治疗所使用的载体系统息息相关。基因载体主要分为病毒载体和非病毒载体两大类:病毒载体的转染效率较高,但副作用较大;非病毒载体作为一种新型的基因传递系统,可以弥补病毒载体的缺陷,尽管其转染效率稍逊于病毒载体,但在基因治疗领域具有不可替代的作用。随着纳米技术的出现和蓬勃发展,基于纳米材料的基因载体研究受到越来越多的关注。纳米基因载体具有如下潜在的优势:它制备相对简单,易于对其进行多功能的修饰;具有良好的生物相容性,一般不会引起强烈的机体免疫反应;粒径普遍很小,容易穿过人体的组织间隙而被细胞吸收,基因转运效率较高;可以较有效保护其所携带外源基因,利于基因更高效地表达。本文主要对基于金属、无机非金属、阳离子聚合物和脂质体纳米材料作为基因载体的研究进展进行综述和展望。

Abstract

Gene therapy has been regarded as a potential treatment for many diseases, including inherited or acquired ones and cancers. Safe, efficient and stable expression of exogenous genes in target cells is the key issue of gene therapy, which depends on the gene delivery system remarkably. Generally, there are two types of gene carriers: viral and non-viral vectors. The transfection efficiency of viral vectors is always higher than that of nonviral ones, but the side effects of viral vectors, such as immune response and random insert of the genes into the host chromosomes, are inevitable during the gene therapy. As the new gene transfer system, non-viral vectors make up for the defects of viral ones and play an irreplaceable role in gene therapy. With the emergence and vigorous development of nanotechnology, vectors based on nanomaterials named nanocarriers have gained more and more attentions for their potential advantages in gene delivery: easily being prepared and multifunctional modified; possessing good biocompatibility and causing less immune response; easily passing through tissues and being absorbed by the target cells with higher gene delivery efficiency; effectively protecting the exogenous genes from being quickly destroyed by the enzymes in tissues or cells. In the review, we mainly focus on the investigations of nanocarriers based on metal, inorganic and non-metal, cationic polymer and liposome nanoparticles and prospect the potential development of the nanocarriers.

Contents
1 Introduction
2 Nanocarriers based on metal nanoparticles
2.1 Magnetic nanoparticles
2.2 Gold nanoparticles
3 Nanocarriers based on inorganic and non-metal nanoparticles
3.1 Carbon nanotubes
3.2 Silica nanoparticles
4 Nanocarriers based on cationic polymers
4.1 Polyethyleneimine nanoparticles
4.2 Chitosan nanoparticles
5 Nanocarriers based on liposomes
6 Conclusion

Graphical abstract

关键词

基因载体 / 纳米基因载体 / 基因治疗 / 转染效率

Key words

gene vector / nanocarrier / gene therapy / transfection efficiency

引用本文

引用格式 ▾

古晓晓, 杜宝吉, 李云辉, 高莹*, 李丹, 汪尔康. 基于纳米材料的基因载体[J]. 化学进展, 2015, 27(8): 1093-1101 DOI:10.7536/PC150163

Gu Xiaoxiao, Du Baoji, Li Yunhui, Gao Ying, Li Dan, Wang Erkang. Vectors Based on Nanomaterials for Gene Delivery[J]. Progress in Chemistry, 2015, 27(8): 1093-1101 DOI:10.7536/PC150163

登录浏览全文

4963

注册一个新账户忘记密码

参考文献

原文顺序 | 出版日期 | 本文引用

[1] Dizaj S M, Jafari S, Khosroushahi A Y. Nanoscale Res. Lett., 2014, 9: 252.
[2] Jolly D. Cancer Gene Ther., 1994, 1: 51.
[3] Rejman J, Oberle V, Zuhorn I, Hoekstra D. Biochem. J., 2004, 377:159.
[4] Davis M E, Zuckerman J E, Choi C H J, Seligson D, Tolcher A, Alabi C A, Yen Y, Heidel J D, Ribas A. Nature, 2010, 464: 1067.
[5] Xu Z P, Zeng Q H, Lu G Q, Yu A B. Chem. Eng. Technol., 2006, 61: 1027.
[6] Laurent S, Forge D, Port M, Roch A, Robic C, Vander Elst L, Muller R N. Chem. Rev., 2008, 108: 2064.
[7] Mah C, Zolotukhin I, Fraites T J, Dobson J, Batich C, Byrne B J. Mol. Ther., 2000, 1: S239.
[8] Xiang J J, Tang J Q, Zhu S G, Nie X M, Lu H B, Shen S R, Li X L, Tang K, Zhou M, Li G Y. J. Gene Med., 2003, 5: 803.
[9] Pan X, Guan J, Yoo J W, Epstein A J, Lee L J, Lee R J. Int. J. Phytorem., 2008, 358: 263.
[10] Zhang Z B, Song L N, Dong J L, Guo D W, Du X L, Cao B Y, Zhang Y, Gu N, Mao X L. J. Nanopart. Res., 2013, 15: 1.
[11] Shi M R, Liu Y Y, Xu M M, Yang H, Wu C H, Miyoshi H. Macromol. Biosci., 2011, 11: 1563.
[12] Rhim W K, Kim J S, Nam J M. Small, 2008, 4: 1651.
[13] Noh S M, Kim W K, Kim S J, Kim J M, Baek K H, Oh Y K. Biochim. Biophys. Acta, 2007, 1770: 747.
[14] Zhou X F, Zhang X Z, Yu X H, Zha X, Fu Q, Liu B, Wang X Y, Chen Y, Chen Y, Shan Y M, Jin Y H, Wu Y G, Liu J Q, Kong W C, Shen J. Biomaterials, 2008, 29: 111.
[15] Hu C, Peng Q, Chen F J, Zhong Z L, Zhuo R X. Bioconjugate Chem., 2010, 21:836.
[16] Ghosh P S, Kim C K, Han G, Forbes N S, Rotello V M. ACS Nano, 2008, 2: 2213.
[17] Li P C, Li D, Zhang L X, Li G P, Wang E K. Biomaterials, 2008, 29: 3617.
[18] Li D, Li P C, Li G P, Wang J, Wang E K. Biomaterials, 2009, 30: 1382.
[19] Du B J, Tian L, Gu X X, Li D, Wang E K, Wang J. Small, DOI: 10.1002/smll.201402470.
[20] Han G, You C, Kim B J, Turingan R S, Forbes N S, Martin C T, Rotello V M. Angew. Chem. Int. Ed., 2006, 45: 3165.
[21] Chen Z Z, Zhang L F, He Y L, Li Y F. ACS Appl. Mater. Inter., 2014, 6: 14196.
[22] Liu Z, Winters Mark, Holodniy Mark, Dai H J. Angew. Chem. Int. Ed., 2007, 46: 2023.
[23] Richard C, Mignet N, Largeau C, Escriou V, Bessodes M, Scherman D. Nano Res., 2009, 2: 638.
[24] Kneuer C, Sameti M, Bakowsky U, Schiestel T, Schirra H, Schmidt H, Lehr C M. Bioconjugate Chem., 2000, 11: 926.
[25] Liu J, Stace-Naughton A, Brinker C J. Chem. Commun., 2009, 14: 5100
[26] Radu D R, Lai C Y, Jeftinija K, Rowe E W, Jeftinija S, Lin Y. J. Am. Chem. Soc., 2004, 126: 13216.
[27] Zhu S G, Lu H B, Xiang J J, Tang K, Zhang B C, Zhou M, Tan C, Li G Y. Chin. Sci. Bull., 2002, 47: 654.
[28] Hartono S B, Gu W Y, Kleitz F, Liu J, He L, Middelberg A P, Yu C, Lu G Q, Qiao S Z. ACS Nano, 2012, 6: 2104.
[29] Ma X, Teh C, Zhang Q, Borah P, Choong C, Korzh V, ZhaoY. Antioxid. Redox. Signal., 2014, 21: 707.
[30] Jin L, Zeng X, Liu M, Deng Y, He N Y. Theranostics, 2014, 4: 240.
[31] Dai Z, Gjetting T, Mattebjerg M A, Wu C, Andresen T L. Biomaterials, 2011, 32: 8626.
[32] Nimesh S, Goyal A, Pawar V, Jayaraman S, Kumar P, Chandra R, Singh Y, Gupta K C. J. Control. Release, 2006, 110: 457.
[33] Nimesh S, Aggarwal A, Kumar P, Singh Y, Gupta K C, Chandra R. Int. J. Pharm., 2007, 337: 265.
[34] Boussif O, Lezoualch F, Zanta M A, Mergny M D, Scherman D, Demeneix B, Behr J P. Proc. Natl. Acad. Sci. U. S. A., 1995, 92: 7297.
[35] Nguyen K C, Muthiah M, Islam M A, Kalash R S, Cho C S, Park H, Lee I K, Kim H J, Park I K, Cho K A. Colloid. Surf. B Bioin., 2014, 119:126.
[36] Chen L, Tian H Y, Chen J, Chen X S, Huang Y B, Jing X B. J. Gene Med., 2010, 12: 64.
[37] Thomas M, Lu J, Zhang C C, Chen J Z, Klibanov A M. Pharm. Res., 2007, 24: 1564.
[38] Bao X L, Wang W, Wang C, Wang Y, Zhou J P, Ding Y, Wang X Y, Jin Y T. Biomaterials, 2014, 35: 8450.
[39] Baginskiy I, Lai T C, Cheng L C, Chan Y C, Yang K Y, Liu R S, Hsiao M, Chen C H, Hu S F, Her L J, Tsai D P, Liu R S. J. Phys. Chem., 2013,117: 2396.
[40] Mumper R J, Wang J J, Claspell J M, Rolland A P. J. Control. Release, 1995, 22: 178.
[41] Danielsen S, Strand S, de Lange D C, Stokke B T. Biochim. Biophys. Acta, 2005, 1721: 44.
[42] Lavertu M, Methot S, Tran-Khanh N, Buschmann M D. Biomaterials, 2006, 27: 4815.
[43] Kiang T, Wen J, Lim H W, Leong K W. Biomaterials, 2004, 25: 5293.
[44] Ishii T, Okahata Y, Sato T. Biochim. Biophys. Acta, 2001, 1514: 51.
[45] Jiang X, Dai H, Leong K W, Goh S H, Mao H Q, Yang Y Y. J. Gene Med., 2006, 8: 477.
[46] Xie Y, Qiao H Z, Su Z G, Chen M L, Ping Q N, Sun M J. Biomaterials, 2014, 35: 7978.
[47] Zhao X, Yin L, Ding J, Tang Cui, Gua S, Yin C, Mao Y. J. Control. Release, 2010, 144: 46.
[48] Felgner P L, Gadek T R, Holm M, Roman R, Chan H W, Wenz M, Northrop J P, Ringold G M, Danielsen M. Proc. Natl. Acad. Sci. U. S. A., 1987, 84: 7413.
[49] Pozzi D, Marchini C, Cardarelli F, Rossetta A, Colapicchioni V, Amici A, Montani M, Motta S, Brocca P, Cantù L, Caracciolo G. Mol. Pharm., 2013, 10: 4654.
[50] Tagalakis A D, He L, Saraiva L, Gustafsson K T, Hart S L. Biomaterials, 2011, 32: 6302.
[51] Writer M J, Kyrtatos P G, Bienemann A S, Pugh J A, Lowe A S, Villegas-Llerena C, Kenny G D, White E A, Gill S S, McLeod C W, Lythgoe M F, Hart S L. J. Control. Release, 2012, 162: 340.
[52] Yan J, Korolev N, Eom K D, Tam J P, Nordenskiold L. Biomacromolecules, 2012, 13: 124.
[53] Ochoa G P, Sesma J Z, Diez M A, Diaz-Tahoces A, Aviles-Trigeros M, Grijalvo S, Eritja R, Fernandez E, Pedraz J L. Pharm. Res., 2014, 31: 1665.
[54] Li P C, Zhang L X, Ai K L, Li D, Liu X H, Wang E K. J. Control. Release, 2008, 129: 128.
[55] Li D, Li G P, Li P C, Zhang L X, Liu Z J, Wang J, Wang E K. Biomaterials, 2010, 31: 1850.