锁核酸分子信标在分子识别与生物分析中的应用

桂珍; 严枫; 李金昌; 葛梦圆; 鞠熀先

doi:10.7536/PC150427

化学进展 ›› 2015, Vol. 27 ›› Issue (10) :1448-1458 DOI: 10.7536/PC150427

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锁核酸分子信标在分子识别与生物分析中的应用

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作者信息 +

Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis

Gui Zhen¹, Yan Feng^1*, Li Jinchang¹, Ge Mengyuan¹, Ju Huangxian²

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文章历史 +

摘要

分子信标是一种荧光探针,闭合时呈发夹结构。其5'末端修饰荧光基团,3'末端修饰猝灭基团。当目标存在时,环部与目标结合,发夹打开,发出荧光。锁核酸是一类双环状寡核苷酸衍生物,能够遵循碱基互补配对原则与核酸结合。锁核酸分子信标技术,结合了分子信标无需分离未结合探针而直接检测的优势和锁核酸亲合力强、热稳定性好、抗酶切以及体内无毒等特点,在核酸检测方面具有灵敏度高、特异性好的独特优势,近年来得到广泛关注。本文介绍了锁核酸修饰分子信标的结构、功能、设计要点,及其研究现状和一些重要进展,并讨论了目前锁核酸分子信标在分子识别及生物分析中的应用及存在的问题和发展前景。

Abstract

Molecular beacons are stem-loop hairpin-structured fluorescence probes with a fluorescent dye at 5' end and a fluorescence quencher at 3' end. When a target complementary sequence is absent, the molecular beacons do not fluoresce, because the formation of the hairpin structure brings the quencher and fluorophore into close proximity, whereby fluorescence is quenched with high efficiency. When a target molecule is present, the hybridization between the target and the loop sequence of the molecular beacon results in the spatial separation of the fluorophore and quencher, which opens the stem-loop structure of molecular beacons to emit fluorescence. Locked nucleic acid is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. It possesses excellent binding affinity to nucleic acid, high biostability and resists to nuclease degradation. Due to the excellent sensitivity and high specificity, the combination of the molecular beacons and locked nucleic acid has aroused wide concern. In this review, we intensively summarize the structure, function, design essentials, current research topics and some important progress. In addition, we also discuss the applications, potential problems and perspective of locked nucleic acid molecular beacons in molecular recognition and bioanalysis.

Contents
1 Introduction
2 Structure and function of molecular beacons
3 Structure and properties of locked nucleic acid
4 Application of LNA in molecular recognition and biomedicine
4.1 LNA antisense
4.2 LNA-modified siRNA (siLNA)
4.3 LNA-modified DNAzyme (LNAzymes)
5 LNA in biotechnology
5.1 LNA primers and PCR
5.2 LNA probes and hybridization
5.3 LNA modification in aptamers
5.4 LNA molecular beacons
6 Design of locked nucleic acid molecular beacons
6.1 Selection of the reporter and quencher
6.2 Hermodynamic aspects
6.3 Balance between selectivity and hybridization rate
6.4 Modification of LNA
7 Applications of LNA-MB in molecular recognition and bioanalysis
7.1 Real time PCR
7.2 mRNA imaging in living cells
7.3 Targeted recognition of microRNA
7.4 Single nucleotide polymorphisms
7.5 Detection of circulating DNA using LNA-MB
8 Conclusion

Graphical abstract

关键词

锁核酸 / 分子信标 / 核酸 / 分子识别 / 生物分析

Key words

locked nucleic acid / molecular beacons / nucleic acids / molecular recognition / bioanalysis

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桂珍, 严枫, 李金昌, 葛梦圆, 鞠熀先. 锁核酸分子信标在分子识别与生物分析中的应用[J]. 化学进展, 2015, 27(10): 1448-1458 DOI:10.7536/PC150427

Gui Zhen, Yan Feng, Li Jinchang, Ge Mengyuan, Ju Huangxian. Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis[J]. Progress in Chemistry, 2015, 27(10): 1448-1458 DOI:10.7536/PC150427

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