基于共轭聚合物的核酸生物传感器的应用

马昀; 周妍; 杜文琦; 缪智辉; 祁争健

doi:10.7536/PC150636

化学进展 ›› 2015, Vol. 27 ›› Issue (12) :1799-1807 DOI: 10.7536/PC150636

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基于共轭聚合物的核酸生物传感器的应用

马昀, 周妍, 杜文琦, 缪智辉, 祁争健*

作者信息 +

The Application of DNA Biosensor Based on Conjugated Polymers

Ma Yun, Zhou Yan, Du Wenqi, Miao Zhihui, Qi Zhengjian*

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文章历史 +

摘要

共轭聚合物的π电子体系及共轭离域结构,使其具有良好的发光性能。聚合物链可充当“分子导线”,能够成倍放大光学信号,从而有效提高检测灵敏度。而核酸适体(aptamer)在特异性、与靶物质亲合力、信号传导方面比其他识别元件具有更大的优势,因此共轭聚合物的核酸生物传感器在生物检测方面得到了迅速发展。本文主要总结了近年来共轭聚合物的核酸生物传感器在生物检测方面的应用,并进一步对该类型传感器的发展趋势作出了展望。

Abstract

Conjugated polymers, with π electron systems and highly delocalized conjugated structures, exhibit excellent luminescence properties. The polymer chains can work as molecular wire, which will lead to the amplification of optical signals and thus improve the detecting sensitivity. Aptamer has advantages in specificity, affinity with targets and signal transmission, hence, nucleic acid biosensors based on conjugated polymers have witnessed a rapid development in bio-detection. The applications of nucleic acid biosensors based on conjugated polymers in recent years are summarized. Finally, an outlook of the developing trend for these sensors is given.

Contents
1 Introduction
2 The sensing mechanism of fluorescent sensor
3 FRET
3.1 Detection of complementary DNA
3.2 Real-time monitoring of DNA hybridization
3.3 Real-time monitoring of DNA structure
3.4 Detection of protein and the activity of enzyme
3.5 Detection of specific gene
4 Aggregation and conformation change
4.1 Detection of complementary DNA
4.2 Real-time monitoring of DNA hybridization
4.3 Real-time monitoring of DNA structure
4.4 Detection of enzyme
4.5 Detection of specific DNA sequence
4.6 Detection of metal ion
5 Superquenching
6 Conclusion

Graphical abstract

关键词

共轭聚合物 / 核酸适体 / 生物检测 / 荧光共振能量转移 / 构象变化

Key words

conjugated polymer / aptamer / biological detection / FRET / conformation change

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马昀, 周妍, 杜文琦, 缪智辉, 祁争健. 基于共轭聚合物的核酸生物传感器的应用[J]. 化学进展, 2015, 27(12): 1799-1807 DOI:10.7536/PC150636

Ma Yun, Zhou Yan, Du Wenqi, Miao Zhihui, Qi Zhengjian*. The Application of DNA Biosensor Based on Conjugated Polymers[J]. Progress in Chemistry, 2015, 27(12): 1799-1807 DOI:10.7536/PC150636

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