光热显微术:基于光吸收的单分子成像技术

袁婷联; 蒋莹琰; 王伟

doi:10.7536/PC160111

化学进展 ›› 2016, Vol. 28 ›› Issue (5) :607-616 DOI: 10.7536/PC160111

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光热显微术:基于光吸收的单分子成像技术

^1,2

^1*

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Photothermal Microscopy: An Absorption-Based Single Molecule Imaging Technology

Yuan Tinglian^1,2, Jiang Yingyan¹, Wang Wei^1*

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摘要

光热显微术是近年来获得广泛关注和长足发展的一种新型光学显微成像技术,能够实现单个纳米粒子甚至单分子的免标记光学成像。其成像原理是利用先进的光学方法探测单分子或单纳米粒子吸收特定波长激发光后所产生的局域温度和介质折射率的微小变化,从而定量研究观测对象的光热特性。由于无辐射弛豫是激发态分子回到基态的优势过程,分子的光热特性相比于荧光特性更具有普遍意义。凭借无需标记、高灵敏度和信号稳定等优点,近十年来,关于单分子和单纳米粒子的光热显微成像研究不断取得突破,并在纳米科学和生命科学等领域获得越来越多的发展和应用,展现出了蓬勃的生命力和良好的发展前景。本文重点综述了光热显微技术的成像原理、发展历程、技术特色以及系统优化方法,列举了光热成像在活细胞研究和生物学领域的应用,最后总结了光热成像的优缺点并分析其主要面临的挑战以及未来的发展趋势,希望吸引更多的研究人员加入到这一新技术的研究队伍中来。

Abstract

Photothermal microscopy (PTM) is an emerging label-free optical microscopy technique that can image the photothermal property of single nanoparticles and single molecules. The image contrast relied on the sensitive detection of local temperature gradients and refractive index distribution associated with the photothermal effect of individual molecules or nanoparticles when it was illuminated with particular incident light. Since non-radiative relaxation is usually the dominant process for an excited molecule to return back to the ground state, photothermal properties are of more generalized significance compared to fluorescence. The past decade has witnessed the great growth and development of photothermal microscopy, mainly because of its advantages of label-free detection, high sensitivity and stability. Furthermore, this technique has received increasing attention in nano-science and life science, ranging from fundamental studies on molecule-photon interactions to promising applications in single cell imaging and bio-sensing. The review mainly describes the imaging principle, optical apparatus, technical concerns for performance optimization of PTM, and subsequently enumerates the important applications in live-cell studies and bioscience. In the last section, we provide perspectives regarding the major strength and challenges for the further development and applications of photothermal microscopy technology.

Contents
1 Introduction
2 Principle and apparatus of PTM
2.1 Imaging principle
2.2 Photothermal interference contrast (PIC)
2.3 Photothermal heterodyne imaging (PHI)
2.4 Performance optimization
3 Imaging single molecules and single nanoparticles
3.1 Plasmonic nanoparticles
3.2 Semiconductor nanocrystals
3.3 Carbon nanotubes
3.4 Organic molecules
4 Application
4.1 Photothermal probes for live cell imaging
4.2 Label-free imaging of biomolecules
4.3 PTM combined with other techniques
5 Conclusion and outlook

Graphical abstract

关键词

光热显微 / 热透镜 / 光热特性 / 单分子成像 / 单纳米粒子成像

Key words

photothermal microscopy / thermal lens / photothermal property / single molecule imaging / single nanoparticle imaging

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袁婷联, 蒋莹琰, 王伟. 光热显微术:基于光吸收的单分子成像技术[J]. 化学进展, 2016, 28(5): 607-616 DOI:10.7536/PC160111

Yuan Tinglian, Jiang Yingyan, Wang Wei. Photothermal Microscopy: An Absorption-Based Single Molecule Imaging Technology[J]. Progress in Chemistry, 2016, 28(5): 607-616 DOI:10.7536/PC160111

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