电化学生物传感应用于体外检测的研究

田亮; 姚琛; 王怡红*

doi:10.7536/PC160908

化学进展 ›› 2016, Vol. 28 ›› Issue (12) :1824-1833 DOI: 10.7536/PC160908

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电化学生物传感应用于体外检测的研究

田亮, 姚琛, 王怡红*

作者信息 +

Recent Advances in Electrochemical Biosensors for In Vitro Diagnostic

Tian Liang, Yao Chen, Wang Yihong*

Author information +

文章历史 +

摘要

电化学生物传感技术以它独特的检测、分析方法以及在临床检测中潜在的应用，近年来受到研究者越来越多的关注。癌症生物标志物的早期检测能够使得患者在癌症发展至晚期前得到治疗，增加患者存活率。此外，生物标志物能够用于确定疾病的复发，以及患者在接受化疗、放疗和外科治疗之后的后续评估。本文主要论述了现有癌症生物标志物检测的设备和方法，并对这些方法的优点和不足作了简单的评述。另外，介绍了体外诊断设备的发展现状和电化学传感技术的特点，并对癌症早期的主要生物标志物进行了介绍，以及着重论述了电化学传感技术应用于临床靶向生物标志物检测的研究进展。此外，还展望了电化学生物传感技术未来的研究方向和发展趋势。从目前的的研究来看，电化学传感技术在体外诊断和临床检测癌症生物标志物等方面存在着较大的应用潜质，有望成为生物、医学、环境等领域重要的研究技术。

Abstract

In recent years, electrochemical biosensor technology has received more and more attention by the virtue of its unique detection, analysis methods and the potential applications in clinical diagnostic. Early detection of cancer biomarkers could diagnose specific diseases timely, and provide treatment for the disease before it develops into its later period to thereby increase survival rate of patients. Furthermore, biomarkers can be used to determine the recurrence of the disease and evaluate the follow-up period after chemotherapy, radiotherapy and surgery. In this paper, we mainly discuss the existing equipment and methods of the cancer biomarkers detection, and briefly comment on the advantages and disadvantages of these methods. In addition, we also introduce the development of in vitro diagnostic devices and the characteristics of electrochemical biosensor technology, and present the major biomarkers in early cancer stage. Furthermore, we also focus on the detection of clinical targeted biomarkers by electrochemical biological sensing technology. The future research direction and development trend of electrochemical biological sensing technology are prospected. According to present researches, electrochemical biological sensing technology possesses great application potential in the areas of in vitro diagnostic and detection of clinical cancer biomarkers. With these features, in vitro diagnostic devices become unique and of great significance, and electrochemical biological sensing technology is expected to be quite important in the field of biology, medicine, environment, and so on.

Contents
1 Introduction
2 Biosensor technology
3 Application of electrochemical biosensor technology
3.1 Electrochemical DNA biosensor
3.2 Electrochemical immunosensor
3.3 Circulating tumor cells (CTCs) electrochemical biosensor
3.4 Glucose electrochemical biosensor
3.5 Hydrogen peroxide electrochemical biosensor
3.6 Electrochemical biosensor for small molecules of metabolite detection
4 Conclusion

Graphical abstract

关键词

体外诊断 / 电化学生物传感 / 蛋白质 / DNA / 循环肿瘤细胞

Key words

in vitro diagnostic / electrochemical biosensor / protein / DNA / circulating tumor cell

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田亮, 姚琛, 王怡红*. 电化学生物传感应用于体外检测的研究[J]. 化学进展, 2016, 28(12): 1824-1833 DOI:10.7536/PC160908

Tian Liang, Yao Chen, Wang Yihong*. Recent Advances in Electrochemical Biosensors for In Vitro Diagnostic[J]. Progress in Chemistry, 2016, 28(12): 1824-1833 DOI:10.7536/PC160908

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