量子点荧光传感器的设计及应用

胡先运; 郭庆生; 刘玉乾; 孙清江; 孟铁宏; 张汝国

doi:10.7536/PC160929

化学进展 ›› 2017, Vol. 29 ›› Issue (2/3) :300-317 DOI: 10.7536/PC160929

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量子点荧光传感器的设计及应用

^1,2*

^1*

作者信息 +

Design Strategies and Applications of Quantum Dots Fluorescent Sensing

Xianyun Hu^1,2*, Qingsheng Guo¹, Yuqian Liu¹, Qingjiang Sun^1*, Tiehong Meng², Ruguo Zhang²

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摘要

量子点（quantum dots，QDs）是一种新型的纳米荧光材料，具有优良的光电性质，已广泛应用于荧光传感及可视化检测，可实现对靶标分子高灵敏、高特异性分析。本文主要论述了量子点的表面化学修饰，以及量子点传感的作用原理，如荧光共振能量转移、电荷转移、直接荧光传感、生物发光共振能量转移、化学发光共振能量转移以及电化学发光，利用这些原理设计出不同的荧光传感器，并应用于不同分子或离子的可视化检测。同时对量子点的荧光传感存在的问题及挑战进行了总结，并提出量子点荧光传感将向生物相容性好、细胞或生物体内实时可视化检测、复杂体系中进行多靶标同时检测以及量子点的逻辑运算等方向发展。

Abstract

Quantum dots (QDs), as novel fluorescent nanomaterials, have been widely used in fluorescent sensing and visual detection with high sensitivity and specificity analysis of target, owing to their excellent optical and electrical properties. The review addresses the surface functionalization of QDs that able to the use of sensing, and discusses different sensing mechanism including fluorescence resonance energy transfer (FRET), electron transfer (CT), direct fluorescent sensing, bioluminescence resonance energy transfer (BRET), chemiluminescence resonance energy transfer (CRET) and electrochemiluminescence (ECL), being applied in the different sensing systems. The challenges and existing problems of QDs fluorescent sensing application are summarized. Meanwhile fluorescent sensing of QDs will develop in the field of the good biocompatibility, real time visualization of sensing in cells or in vivo, multiplexed detection in complex systems and the function of sensing with logic-gate operations in the future.

Contents
1 Introduction
2 Photo-properties of quantum dots
3 Surface chemistry and conjugates of quantum dots
4 Mechanisms and design strategies of quantum dots fluorescence sensing
4.1 Fluorescence resonance energy transfer
4.2 Electron transfer
4.3 Direct fluorescent sensing
4.4 Bioluminescence resonance energy transfer
4.5 Chemiluminescence resonance energy transfer
4.6 Electrochemiluminescence
5 Conclusion

Graphical abstract

关键词

量子点 / 荧光传感 / 探针 / 可视化检测

Key words

quantum dots / fluorescent sensing / probe / visualization detection

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胡先运, 郭庆生, 刘玉乾, 孙清江, 孟铁宏, 张汝国. 量子点荧光传感器的设计及应用[J]. 化学进展, 2017, 29(2/3): 300-317 DOI:10.7536/PC160929

Xianyun Hu, Qingsheng Guo, Yuqian Liu, Qingjiang Sun, Tiehong Meng, Ruguo Zhang. Design Strategies and Applications of Quantum Dots Fluorescent Sensing[J]. Progress in Chemistry, 2017, 29(2/3): 300-317 DOI:10.7536/PC160929

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