高分子类型MONOLITH材料（也称整体柱，连续床）是近些年来发展迅速的一种新型的以高分子为基质的整体型多孔材料，由于其内部独特的三维连续相互贯通的多孔结构，在诸多应用领域越来越受到研究者的关注，尤其被认为是分离过滤领域的一个历史性突破。与硅基质MONOLITH材料相比，高分子类型的MONOLITH材料具有制备工艺简单、生物相容性好、化学稳定性高和表面化学性质易调控等特点，因此作为亲和色谱的固定相在生物大分子的分离分析上具有更大的优势。本综述重点总结了高分子类型MONOLITH材料的制备方法及其最新研究进展，并论述了近5年来其作为亲和色谱固定相用于分离生物大分子的应用进展。
In recent years, a newly emerging polymer-based MONOLITH material (also known as the monolithic column, continuous bed) has been developed rapidly and it has already been utilized widely in various fields. Due to its unique three-dimensional continuous interconnected porous structure in a single piece, polymer-based MONOLITH has attracted more and more attention and it is regarded as one of the major breakthroughs in separation and filtration techniques. Compared with silica-based MONOLITH material, polymer-based MONOLITH material has the advantages of simple preparation process, good biocompatibility, high chemical stability and easy regulation of surface chemical properties. Therefore, polymer-based MONOLITH has always done a better job as a stationary phase of affinity chromatography for separating and analyzing biological macromolecules. In this review, the preparation techniques and the newest development of the polymer-based MONOLITH material are summarized. In addition, its application as stationary phase of affinity chromatography for the separation of biological macromolecules in the last 5 years is described.
Contents
1 Introduction
2 Preparation techniques of the polymer-based MONOLITH materials
2.1 Free radical polymerization
2.2 Controlled/living radical polymerization
2.3 Polymerized high internal phase emulsions
2.4 Condensation polymerization
2.5 Cryogels
2.6 Other methods
3 Application of the polymer-based MONOLITH materials in separation of biomolecules by affinity chromatography
3.1 Bioaffinity chromatography
3.2 Immunoaffinity chromatography
3.3 Dye affinity chromatography
3.4 Boronate affinity chromatography
3.5 Immobilized metal-ion affinity chromatography
3.6 Pseudo affinity chromatography
4 Conclusion
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