Preparation of new functional materials by self-assembly technique has become a hot research field of polymeric materials science. Most of the amphiphilic polymers, such as block copolymers, graft copolymers, star copolymers, dendrimers, part of the random copolymer and polyelectrolyte, etc. can self-assemble under certain conditions. In the study of polymer self-assembly, fluorescent technique has been widely used, especially fluorescent probe technique. According to the changes of the characteristic fluorescence parameters of the probe molecules, such as wavelength, intensity, polarization, lifetime, etc. the critical micelle concentration, temperature and pH dependence, the relationship of structure and self-assemble morphology of the polymer could be easily and accurately studied. This paper is focused on the application of the fluorescent probe technique in investigation of self-assembly behavior of amphiphilic polymers. The effects of hydrophilic lipophilic balance (HLB), concentration, temperature, pH, solvent composition, ionic strength, etc. on self-assemble morphology and microscopic characteristics parameters of amphiphilic polymers are particularly reviewed. Furthermore, based on our own research work, the applications of intrinsic fluorescence spectroscopy method in polymer investigation are elaborated. It will characterize the conformation transitions of macromolecules during the self-assembly process more truly. This paper aims at providing reference for design, polymerization, self-assembly controlling and applications of amphiphilic polymers.

Contents
1 Introduction
2 Several major fluorescence probe molecules
3 The effect of the hydrophilic lipophilic balance (HLB) on polymer self-assembly behavior studied by fluorescence probe technique
4 The effect of temperature on polymer self-assembly behavior investigated by fluorescence probe technique
5 The effect of pH on polymer self-assembly behavior investigated by fluorescence probe technique
6 Application of fluorescent probe technique in other polymer self-assembly behavior investigation
7 Self-assembly of polyelectrolyte behavior investigated by fluorescence probe technique
8 Investigation of polymer conformational transition by fluoresence technique
9 Conclusion and outlook