Microfluidic paper-based chip analysis is a burgeoning microfluidic technique. It possesses great potentials for application in clinical diagnosis, food quality control as well as environmental monitoring due to its attractive features such as low-cost, easy-to-fabricate, easy-to-use and portable.Its importance and utility are widely acknowledged and extensive research has been conducted in the past several years. This paper mainly aims to review the developed techniques for fabrication of microfluidic paper-based chips, including UV photolithography, wax printing, plasma treatment, ink printing, ink jet etching, plotting, screen printing, flexography printing and laser treatment, and so on. The detection methods for the microfluidic paper-based chip analysis and applications of microfluidic paper-based chips are also reviewed.

Contents
1 Introduction
2 Paper choices
3 Fabrication techniques of microfluidic paper-based chips
3.1 UV photolithography technique
3.2 Wax printing technique
3.3 Plasma treatment technique
3.4 Ink printing technique
3.5 Ink jet etching technique
3.6 Plotting technique
3.7 Screen printing and flexography printing technique
3.8 Wax dipping technique
3.9 Laser treatment technique
3.10 Other techniques
4 Detection methods in microfluidic paper-based analytical devices
4.1 Colorimetric detection
4.2 Electrochemical detection
4.3 Chemiluminescence and electrochemilumine-scence detection
5 Applications of microfluidic paper-based analytical devices
5.1 Clinical diagnosis
5.2 Food quality control
5.3 Environmental monitoring
6 Conclusion and perspective