Surface plasmon resonance (SPR) biosensor technology, with countless researches and attractive progress, has been commonly applied in the field of biochemical analysis. From theoretical research to design and produce of bioanalytical instrument, application development and performance improvement, our research team has been engaged in SPR study for almost 10 years and always concerned about the latest progress. This paper mainly includes: the description of principle, components and accessories of SPR bioanalytical instrument, modulation types and coupling configurations of SPR sensor, as well as SPR imaging sensor; the methods for improving the bioanalytical instrument performance, namely, the combination with local surface plasmon resonance (LSPR) technology, metal film redesign and data processing optimization; the lasted developments, involving the technology combination between the SPR and microfluidic chip, electrochemistry, surface-enhanced raman scattering (SERS); the applications in clinical diagnosis, drugscreening, human biomolecular research, food safety and environmental monitoring; and the main problems and future trends of SPR bioanalytical instrument.

Contents
1 Introduction
2 SPR bioanalytical instrument
2.1 Principle of SPR
2.2 SPR instrument
3 SPR sensors
3.1 Modulation types
3.2 Coupling configurations
3.3 SPR imaging sensors
4 Performance improvement mothods of SPR biosensors
4.1 Local surface plasmon resonance
4.2 Metal film
4.3 Data processing
5 The developments of SPR biosensors
5.1 SPR combined with microfluidic chip
5.2 SPR combined with electrochemical
5.3 SPR combined with SERS
6 Applications
7 Conclusions and prospects