Surface plasmon resonance sensing and imaging methods have been developing very fast during recent years, especially in applying to the study of biomolecular interactions and/or recognitions and determination of kinetic and thermodynamic constants. These progresses are broadly interesting but remain short of reviewing. This paper is thus designed and dedicated to the methodization of the very recent, critical developments in the study of molecular reaction kinetics and measure of their constants. In the end, their prospect is given, in addition to a brief summary of other research progresses.

Contents
1 Introduction
2 Basic principles
2.1 SPR/SPRi principle
2.2 Kinetics principle
2.3 SPR/SPRi approach
2.4 Parameter calculation
3 Practical Methods
3.1 Multi-cycle
3.2 Improved multi-cycle
3.3 Kinetic titration
3.4 Steady state
3.5 Arraying
4 Conclusion and prospect