Microfluidic system integrated with different functional units is widely employed in biochemical analysis and shows great potentials compared with conventional approaches for bacteria detection. Recently, novel nano fluorescent probes with improved fluorescent properties, low background and enhanced selectivity provide an efficient way for pathogenic bacteria quantitation, especially when they are combined with miniaturized platform or microfluidic device. Several novel nano fluorescent probes for bacteria detection are introduced and discussed in this review. Moreover, special attention has been paid to the recent research about combination of these approaches in bacteria analysis using variety of nano fluorescent probes on the microfluidic chip. It is also focused that the development of effective bacteria detection under the micro scale space with fluorescent monitoring mode.

Contents
1 Introduction
2 The technology of bacteria detection on microfluidic chips
3 New nano-fluorescent probes for bacteria labeled and detection
4 Detection of bacteria by nano-fluorescent probes on microfluidic chip
4.1 Detection of bacteria with nano-fluorescent probes
4.2 Detection of bacteria with nano-fluorescent probes and aptamers
4.3 Detection of bacteria with nano-fluorescent probes and immunoassay
5 Conclusion and perspective