In recent years, electrochemical biosensor technology has received more and more attention by the virtue of its unique detection, analysis methods and the potential applications in clinical diagnostic. Early detection of cancer biomarkers could diagnose specific diseases timely, and provide treatment for the disease before it develops into its later period to thereby increase survival rate of patients. Furthermore, biomarkers can be used to determine the recurrence of the disease and evaluate the follow-up period after chemotherapy, radiotherapy and surgery. In this paper, we mainly discuss the existing equipment and methods of the cancer biomarkers detection, and briefly comment on the advantages and disadvantages of these methods. In addition, we also introduce the development of in vitro diagnostic devices and the characteristics of electrochemical biosensor technology, and present the major biomarkers in early cancer stage. Furthermore, we also focus on the detection of clinical targeted biomarkers by electrochemical biological sensing technology. The future research direction and development trend of electrochemical biological sensing technology are prospected. According to present researches, electrochemical biological sensing technology possesses great application potential in the areas of in vitro diagnostic and detection of clinical cancer biomarkers. With these features, in vitro diagnostic devices become unique and of great significance, and electrochemical biological sensing technology is expected to be quite important in the field of biology, medicine, environment, and so on.

Contents
1 Introduction
2 Biosensor technology
3 Application of electrochemical biosensor technology
3.1 Electrochemical DNA biosensor
3.2 Electrochemical immunosensor
3.3 Circulating tumor cells (CTCs) electrochemical biosensor
3.4 Glucose electrochemical biosensor
3.5 Hydrogen peroxide electrochemical biosensor
3.6 Electrochemical biosensor for small molecules of metabolite detection
4 Conclusion