The surface design of biomedical materials is becoming more and more important in the fields of tissue engineering, biomedical devices, biosensing and detection, and biochips. In-depth understanding of the properties of materials, especially the surface properties, has a certain guiding role in the development of biomedical materials. Among various methods of surface modification based on biomaterials, photochemical modification is very simple and efficient, and has the advantages of spatiotemporal controllability and non-invasiveness. Based on this, in recent years, photochemical modification has become one of the hot research fields in the interface modification of biomaterials. This review first simply introduces the recent development of photochemical modification methods on biochemical surface, then mainly focuses on tissue regeneration materials(extracellular matrix biomimetic surfaces, stiffness-controllable hydrogel films, patterning and gradient surfaces, photo-responsive smart surfaces), 2D droplet microarrays surfaces, high-throughput biochip and other applications. Finally, this article points out the current shortcomings of photochemical surface modification and the future development trend of this field is also proposed.