Bioactive peptides play an important role in physiological processes. Accurate analysis of bioactive peptides will help to further develop their efficacy. Due to the bioactive peptides often coexisting with high concentrations of proteins, it is still quite difficult to analyze peptides in complex biological systems. These coexisted proteins may severely reduce the separation efficiency of chromatographic column for bioactive peptides and inhibit the peak signal of the peptide in the mass spectrum. In view of this, metal-organic frameworks are introduced to perform enrichment analysis of active peptides. Metal-organic frameworks(MOFs) is a type of framework self-assembled by coordination bonds between metal ions or metal clusters and organic ligands to form organic-inorganic hybrid materials with porous structure. Due to their adjustable frame structure, high porosity, good chemical stability, reproducibility, and simple synthesis process, they are widely used in active peptides enrichment, gas adsorption and separation, the areas of sensors, drug delivery and catalytic reactions. In this paper, the research progress of MOF materials as active peptide enrichment materials such as phosphopeptides, glycopeptides and endogenous peptides in recent years is reviewed, and the shortcomings and outlook of MOFs materials in this area are pointed out.