CRISPR (Clustered regularly interspaced short palindromic repeats) technology is a revolutionary gene editing tool developed in recent years, which has quickly become a cutting-edge technology in the field of biology and medicine and widely used in gene function research and gene therapy. In addition to excellent gene editing capabilities, various new functions are also being continuously created with the efforts of scientists from different fields. CRISPR has excellent sequence recognition properties, nucleic acid cleavage ability, and is easy to program and design. Therefore, it exhibits unique charm in the field of bioanalytical chemistry, and has made breakthrough progress in virus detection, clinical diagnosis, and single cell imaging analysis. At present, there are many types of detection methods using CRISPR technology. Therefore, we summarize and classify the current research status of CRISPR analysis and detection methods, and look forward to its development trend.