Covalent organic frameworks(COFs) are a class of crystalline porous organic material, constructed with light elements by reversible covalent bonds. Due to their high surface area, low density, regular channel structure and facile functionalization, COFs have attracted much attention and shown high perspectives in gas adsorption, chemical sensing, heterogeneous catalysis, etc. Recently, COFs have shown potential applications in enzyme immobilization and mimic enzymes. COFs present an attractive category of enzyme immobilization matrix, because the functional groups on COFs can be readily tailored to hold specific interactions between COFs and enzymes. Moreover, the continuous and confined open channels of COFs provide a favorable micro-environment for infiltrating enzymes. Meanwhile, the mimic enzyme features of COFs are explored, COF mimic enzymes are designed either by “from bottom to top” method or post modification strategy. As a result, not only the carrier materials for enzyme immobilization are expanded, but also it provides new ideas for biomimetic catalysis of mimic enzymes. This review focuses on recent advances of COFs immobilized enzyme and COFs mimic enzymes(nanozyme) applied in biocatalysis. Special emphasis is placed on the deliberation of synthetic and functional strategies, immobilization methods of COFs carrier, as well as the design concept, catalytic activity and selectivity of COFs mimic enzymes. Finally, the remaining challenges of COFs in enzyme catalysis and prospects in this field are summarized.