Biological Condensed Matters(BCM), which are composed of proteins, nucleic acids, polysaccharides and other Biological macromolecules, are ubiquitous inside the organism. These BCM form various structures to perform different function. The determination of the high-resolution structures of these BCM is importance of understanding the life process. In Vitro, X-ray crystallography, cryo-electron microscopy(cryo-EM) and nuclear magnetic resonance(NMR) are the main methods to obtain high-resolution structures, while nuclear magnetic resonance and chemical cross-linking mass spectrometry have unique advantages for in situ study of the structure of BCM in living cells. Here, we mainly summarize the research progress in the characterization of the structures of BCM: including purified molecular machines and fibrils, biomolecules under crowding, confinement, liquid-liquid phase separation and in living cells.